diff --git a/CMakeLists.txt b/CMakeLists.txt
index 85cb6ca7b4..153d304183 100644
--- a/CMakeLists.txt
+++ b/CMakeLists.txt
@@ -227,9 +227,10 @@ endif()
 # - set HAVE_XXX
 
 #The minimum package list
-set(PACKAGELIST GLIB2 GMODULE2 M LEX YACC Perl SH PythonInterp)
+set(PACKAGELIST GLIB2 GMODULE2 GTHREAD2 M LEX YACC Perl SH PythonInterp)
 set(GLIB2_FIND_REQUIRED)
 set(GLIB2_MIN_VERSION 2.14.0)
+set(GTHREAD2_REQUIRED)
 set(PCAP_REQUIRED)
 set(M_REQUIRED)
 
@@ -249,14 +250,6 @@ if(BUILD_wireshark)
 		set(GTK2_FIND_VERSION 2.12)
 		set(GTK2_DEBUG false)
 	endif()
-	if(ENABLE_THREADS)
-		set(PACKAGELIST ${PACKAGELIST} GTHREAD2)
-	endif()
-endif()
-
-if(BUILD_dumpcap)
-	set(PACKAGELIST ${PACKAGELIST} GTHREAD2)
-	set(GHTREAD2_REQUIRED)
 endif()
 
 # SMI SNMP
diff --git a/CMakeOptions.txt b/CMakeOptions.txt
index ea43c4ab4f..7f587378b5 100644
--- a/CMakeOptions.txt
+++ b/CMakeOptions.txt
@@ -31,7 +31,6 @@ option(ENABLE_STATIC     "Build a static version of Wireshark (not yet working)"
 option(ENABLE_PLUGINS    "Build with plugins" ON)
 option(ENABLE_GUIDES     "Build Guides" OFF)
 option(ENABLE_PCAP_NG_DEFAULT	"Enable pcap-ng as default file format" ON)
-option(ENABLE_THREADS    "Build with threads support" ON)
 
 option(ENABLE_ADNS       "Build with adns support" ON)
 option(ENABLE_PORTAUDIO  "Build with portaudio support" ON)
@@ -46,4 +45,3 @@ option(ENABLE_CAP        "Build with posix capabilities support" ON)
 option(ENABLE_CARES      "Build with c_ares support" ON)
 # todo Mostly hardcoded
 option(ENABLE_KERBEROS   "Build with Kerberos support" ON)
-
diff --git a/cmakeconfig.h.in b/cmakeconfig.h.in
index 0ef4163680..2a4e1ab644 100644
--- a/cmakeconfig.h.in
+++ b/cmakeconfig.h.in
@@ -18,9 +18,6 @@
 /* Directory where plugins reside */
 #define PLUGIN_DIR "${CMAKE_INSTALL_PREFIX}/${CMAKE_INSTALL_LIBDIR}/@CPACK_PACKAGE_NAME@/plugins/${CPACK_PACKAGE_VERSION}"
 
-/* Define to 1 if we want to use threads in wireshark and dumpcap */
-#cmakedefine USE_THREADS 1
-
 /* Define to 1 if we want to use pcap-ng as our default file format */
 #cmakedefine PCAP_NG_DEFAULT 1
 
diff --git a/config.h.win32 b/config.h.win32
index 7055cf931a..fa7e6bff18 100644
--- a/config.h.win32
+++ b/config.h.win32
@@ -29,9 +29,6 @@
 #define UNICODE 1
 #define _UNICODE 1
 
-/* Use threads */
-#define USE_THREADS 1
-
 /* Define if you have the ANSI C header files.  */
 #define STDC_HEADERS 1
 
diff --git a/configure.in b/configure.in
index 0b65f60a4b..0314b58b98 100644
--- a/configure.in
+++ b/configure.in
@@ -746,14 +746,6 @@ if test x$enable_packet_editor = xyes; then
 	AC_DEFINE(WANT_PACKET_EDITOR, 1, [Support for packet editor])
 fi
 
-AC_ARG_ENABLE(threads,
-  AC_HELP_STRING( [--enable-threads],
-                  [use threads in Wireshark @<:@default=yes@:>@]),
-    enable_threads=$enableval,enable_threads=yes)
-if test x$enable_threads = xyes; then
-	AC_DEFINE(USE_THREADS, 1, [Support for threads])
-fi
-
 AC_ARG_ENABLE(profile-build,
   AC_HELP_STRING( [--enable-profile-build],
 		  [build profile-ready binaries @<:@default=no@:>@]),
@@ -2140,7 +2132,6 @@ echo "                        Use plugins : $have_plugins"
 echo "                    Use Lua library : $lua_message"
 echo "                 Use Python binding : $python_message"
 echo "                   Build rtp_player : $portaudio_message"
-echo "                        Use threads : $enable_threads"
 echo "             Build profile binaries : $enable_profile_build"
 echo "                   Use pcap library : $want_pcap"
 echo "                   Use zlib library : $zlib_message"
diff --git a/dumpcap.c b/dumpcap.c
index eb97f1e19b..88699edd20 100644
--- a/dumpcap.c
+++ b/dumpcap.c
@@ -235,7 +235,7 @@ typedef struct _pcap_options {
 #endif
     gboolean       cap_pipe_modified;     /* TRUE if data in the pipe uses modified pcap headers */
     gboolean       cap_pipe_byte_swapped; /* TRUE if data in the pipe is byte swapped */
-#if defined(USE_THREADS) && defined(_WIN32)
+#if defined(_WIN32)
     char *         cap_pipe_buf;          /* Pointer to the data buffer we read into */
 #endif
     int            cap_pipe_bytes_to_read;/* Used by cap_pipe_dispatch */
@@ -247,7 +247,7 @@ typedef struct _pcap_options {
         STATE_READ_DATA
     } cap_pipe_state;
     enum { PIPOK, PIPEOF, PIPERR, PIPNEXIST } cap_pipe_err;
-#if defined(USE_THREADS) && defined(_WIN32)
+#if defined(_WIN32)
     GMutex *cap_pipe_read_mtx;
     GAsyncQueue *cap_pipe_pending_q, *cap_pipe_done_q;
 #endif
@@ -318,7 +318,7 @@ static gboolean need_timeout_workaround;
  * WaitForSingleObject. If it's less than 1000 WaitForSingleObject
  * will return immediately.
  */
-#if defined(USE_THREADS) && defined(_WIN32)
+#if defined(_WIN32)
 #define PIPE_READ_TIMEOUT   100000
 #else
 #define PIPE_READ_TIMEOUT   250000
@@ -415,9 +415,7 @@ print_usage(gboolean print_ver)
     fprintf(output, "  -n                       use pcapng format instead of pcap\n");
     fprintf(output, "\n");
     fprintf(output, "Miscellaneous:\n");
-#ifdef USE_THREADS
     fprintf(output, "  -t                       use a separate thread per interface\n");
-#endif
     fprintf(output, "  -q                       don't report packet capture counts\n");
     fprintf(output, "  -v                       print version information and exit\n");
     fprintf(output, "  -h                       display this help and exit\n");
@@ -1547,7 +1545,7 @@ cap_pipe_adjust_header(gboolean byte_swapped, struct pcap_hdr *hdr, struct pcapr
     }
 }
 
-#if defined(USE_THREADS) && defined(_WIN32)
+#if defined(_WIN32)
 /*
  * Thread function that reads from a pipe and pushes the data
  * to the main application thread.
@@ -1635,7 +1633,7 @@ static void *cap_pipe_read(void *arg)
 }
 #endif
 
-#if (!(defined(USE_THREADS) && defined(_WIN32))) || defined(MUST_DO_SELECT)
+#if !defined(_WIN32) || defined(MUST_DO_SELECT)
 /* Provide select() functionality for a single file descriptor
  * on UNIX/POSIX. Windows uses cap_pipe_read via a thread.
  *
@@ -1682,7 +1680,7 @@ cap_pipe_open_live(char *pipename,
     wchar_t *err_str;
 #endif
 #endif
-#if !(defined(USE_THREADS) && defined(_WIN32))
+#ifndef _WIN32
     int          sel_ret;
     unsigned int bytes_read;
 #endif
@@ -1851,7 +1849,7 @@ cap_pipe_open_live(char *pipename,
 
     pcap_opts->from_cap_pipe = TRUE;
 
-#if !(defined(USE_THREADS) && defined(_WIN32))
+#ifndef _WIN32
     /* read the pcap header */
     bytes_read = 0;
     while (bytes_read < sizeof magic) {
@@ -1926,7 +1924,7 @@ cap_pipe_open_live(char *pipename,
         goto error;
     }
 
-#if !(defined(USE_THREADS) && defined(_WIN32))
+#ifndef _WIN32
     /* Read the rest of the header */
     bytes_read = 0;
     while (bytes_read < sizeof(struct pcap_hdr)) {
@@ -2006,7 +2004,7 @@ cap_pipe_dispatch(loop_data *ld, pcap_options *pcap_opts, guchar *data, char *er
     struct pcap_pkthdr phdr;
     enum { PD_REC_HDR_READ, PD_DATA_READ, PD_PIPE_EOF, PD_PIPE_ERR,
            PD_ERR } result;
-#if defined(USE_THREADS) && defined(_WIN32)
+#ifdef _WIN32
     GTimeVal wait_time;
     gpointer q_status;
 #else
@@ -2023,7 +2021,7 @@ cap_pipe_dispatch(loop_data *ld, pcap_options *pcap_opts, guchar *data, char *er
     switch (pcap_opts->cap_pipe_state) {
 
     case STATE_EXPECT_REC_HDR:
-#if defined(USE_THREADS) && defined(_WIN32)
+#ifdef _WIN32
         if (g_mutex_trylock(pcap_opts->cap_pipe_read_mtx)) {
 #endif
 
@@ -2032,7 +2030,7 @@ cap_pipe_dispatch(loop_data *ld, pcap_options *pcap_opts, guchar *data, char *er
                 sizeof(struct pcaprec_modified_hdr) : sizeof(struct pcaprec_hdr);
             pcap_opts->cap_pipe_bytes_read = 0;
 
-#if defined(USE_THREADS) && defined(_WIN32)
+#ifdef _WIN32
             pcap_opts->cap_pipe_buf = (char *) &pcap_opts->cap_pipe_rechdr;
             g_async_queue_push(pcap_opts->cap_pipe_pending_q, pcap_opts->cap_pipe_buf);
             g_mutex_unlock(pcap_opts->cap_pipe_read_mtx);
@@ -2041,7 +2039,7 @@ cap_pipe_dispatch(loop_data *ld, pcap_options *pcap_opts, guchar *data, char *er
         /* Fall through */
 
     case STATE_READ_REC_HDR:
-#if !(defined(USE_THREADS) && defined(_WIN32))
+#ifndef _WIN32
         b = read(pcap_opts->cap_pipe_fd, ((char *)&pcap_opts->cap_pipe_rechdr)+pcap_opts->cap_pipe_bytes_read,
                  pcap_opts->cap_pipe_bytes_to_read - pcap_opts->cap_pipe_bytes_read);
         if (b <= 0) {
@@ -2073,7 +2071,7 @@ cap_pipe_dispatch(loop_data *ld, pcap_options *pcap_opts, guchar *data, char *er
         break;
 
     case STATE_EXPECT_DATA:
-#if defined(USE_THREADS) && defined(_WIN32)
+#ifdef _WIN32
         if (g_mutex_trylock(pcap_opts->cap_pipe_read_mtx)) {
 #endif
 
@@ -2081,7 +2079,7 @@ cap_pipe_dispatch(loop_data *ld, pcap_options *pcap_opts, guchar *data, char *er
             pcap_opts->cap_pipe_bytes_to_read = pcap_opts->cap_pipe_rechdr.hdr.incl_len;
             pcap_opts->cap_pipe_bytes_read = 0;
 
-#if defined(USE_THREADS) && defined(_WIN32)
+#ifdef _WIN32
             pcap_opts->cap_pipe_buf = (char *) data;
             g_async_queue_push(pcap_opts->cap_pipe_pending_q, pcap_opts->cap_pipe_buf);
             g_mutex_unlock(pcap_opts->cap_pipe_read_mtx);
@@ -2090,7 +2088,7 @@ cap_pipe_dispatch(loop_data *ld, pcap_options *pcap_opts, guchar *data, char *er
         /* Fall through */
 
     case STATE_READ_DATA:
-#if !(defined(USE_THREADS) && defined(_WIN32))
+#ifndef _WIN32
         b = read(pcap_opts->cap_pipe_fd, data+pcap_opts->cap_pipe_bytes_read,
                  pcap_opts->cap_pipe_bytes_to_read - pcap_opts->cap_pipe_bytes_read);
         if (b <= 0) {
@@ -2292,14 +2290,14 @@ capture_loop_open_input(capture_options *capture_opts, loop_data *ld,
 #endif
         pcap_opts->cap_pipe_modified = FALSE;
         pcap_opts->cap_pipe_byte_swapped = FALSE;
-#if defined(USE_THREADS) && defined(_WIN32)
+#ifdef _WIN32
         pcap_opts->cap_pipe_buf = NULL;
 #endif
         pcap_opts->cap_pipe_bytes_to_read = 0;
         pcap_opts->cap_pipe_bytes_read = 0;
         pcap_opts->cap_pipe_state = 0;
         pcap_opts->cap_pipe_err = PIPOK;
-#if defined(USE_THREADS) && defined(_WIN32)
+#ifdef _WIN32
         pcap_opts->cap_pipe_read_mtx = g_mutex_new();
         pcap_opts->cap_pipe_pending_q = g_async_queue_new();
         pcap_opts->cap_pipe_done_q = g_async_queue_new();
@@ -2636,7 +2634,7 @@ capture_loop_dispatch(loop_data *ld,
     int       inpkts;
     gint      packet_count_before;
     guchar    pcap_data[WTAP_MAX_PACKET_SIZE];
-#if !(defined(USE_THREADS) && defined(_WIN32))
+#ifndef _WIN32
     int       sel_ret;
 #endif
 
@@ -2646,7 +2644,7 @@ capture_loop_dispatch(loop_data *ld,
 #ifdef LOG_CAPTURE_VERBOSE
         g_log(LOG_DOMAIN_CAPTURE_CHILD, G_LOG_LEVEL_DEBUG, "capture_loop_dispatch: from capture pipe");
 #endif
-#if !(defined(USE_THREADS) && defined(_WIN32))
+#ifndef _WIN32
         sel_ret = cap_pipe_select(pcap_opts->cap_pipe_fd);
         if (sel_ret <= 0) {
             if (sel_ret < 0 && errno != EINTR) {
@@ -2664,7 +2662,7 @@ capture_loop_dispatch(loop_data *ld,
             if (inpkts < 0) {
                 ld->go = FALSE;
             }
-#if !(defined(USE_THREADS) && defined(_WIN32))
+#ifndef _WIN32
         }
 #endif
     }
@@ -3856,10 +3854,8 @@ main(int argc, char *argv[])
     global_ld.pcaps = g_array_new(FALSE, FALSE, sizeof(pcap_options *));
 
     /* Initialize the thread system */
-#ifdef USE_THREADS
-    if (!g_thread_supported())
-        g_thread_init(NULL);
-#endif
+    g_thread_init(NULL);
+
 #ifdef _WIN32
     /* Load wpcap if possible. Do this before collecting the run-time version information */
     load_wpcap();
@@ -4076,11 +4072,9 @@ main(int argc, char *argv[])
         case 'q':        /* Quiet */
             quiet = TRUE;
             break;
-#ifdef USE_THREADS
         case 't':
             use_threads = TRUE;
             break;
-#endif
             /*** all non capture option specific ***/
         case 'D':        /* Print a list of capture devices and exit */
             list_interfaces = TRUE;
@@ -4148,12 +4142,10 @@ main(int argc, char *argv[])
     } else {
         /* We're supposed to capture traffic; */
         /* Are we capturing on multiple interface? If so, use threads and pcapng. */
-#ifdef USE_THREADS
         if (global_capture_opts.ifaces->len > 1) {
             use_threads = TRUE;
             global_capture_opts.use_pcapng = TRUE;
         }
-#endif
         /* Was the ring buffer option specified and, if so, does it make sense? */
         if (global_capture_opts.multi_files_on) {
             /* Ring buffer works only under certain conditions:
diff --git a/epan/prefs.c b/epan/prefs.c
index 8e21a8359e..4bb7e15e09 100644
--- a/epan/prefs.c
+++ b/epan/prefs.c
@@ -2410,9 +2410,6 @@ set_pref(gchar *pref_name, gchar *value, void *private_data _U_,
   } else if (strcmp(pref_name, PRS_CAP_SHOW_INFO) == 0) {
     prefs.capture_show_info = ((g_ascii_strcasecmp(value, "true") == 0)?TRUE:FALSE);
   } else if (strcmp(pref_name, PRS_CAP_SYNTAX_CHECK_FILTER) == 0) {
-#ifndef USE_THREADS
-    prefs.capture_syntax_check_filter = ((g_ascii_strcasecmp(value, "true") == 0)?TRUE:FALSE);
-#endif
 /* handle the global options */
   } else if (strcmp(pref_name, PRS_NAME_RESOLVE) == 0 ||
 	     strcmp(pref_name, PRS_CAP_NAME_RESOLVE) == 0) {
@@ -3359,13 +3356,6 @@ write_prefs(char **pf_path_return)
   fprintf(pf, PRS_CAP_SHOW_INFO ": %s\n",
 	  prefs.capture_show_info == TRUE ? "TRUE" : "FALSE");
 
-#ifndef USE_THREADS
-  fprintf(pf, "\n# Syntax check capture filter?\n");
-  fprintf(pf, "# TRUE or FALSE (case-insensitive).\n");
-  fprintf(pf, PRS_CAP_SYNTAX_CHECK_FILTER ": %s\n",
-	  prefs.capture_syntax_check_filter == TRUE ? "TRUE" : "FALSE");
-#endif
-
   fprintf (pf, "\n######## Printing ########\n");
 
   fprintf (pf, "\n# Can be one of \"text\" or \"postscript\".\n"
diff --git a/gtk/capture_dlg.c b/gtk/capture_dlg.c
index 89c587d67c..ded8a320f5 100644
--- a/gtk/capture_dlg.c
+++ b/gtk/capture_dlg.c
@@ -293,12 +293,10 @@ typedef struct capture_filter_check {
 /* We could make this smarter by caching results */
 capture_filter_check_t cfc_data;
 
-#ifdef USE_THREADS
 GMutex *pcap_compile_mtx = NULL;
 GCond *cfc_data_cond = NULL;
 GMutex *cfc_data_mtx = NULL;
 GThread *cfc_thread = NULL;
-#endif
 
 #if 0
 #define DEBUG_SYNTAX_CHECK(state1, state2) g_warning("CF state %s -> %s : %s", state1, state2, cfc_data.filter_text)
@@ -311,28 +309,25 @@ check_capture_filter_syntax(void *data _U_) {
   struct bpf_program fcode;
   int pc_err;
 
-#ifdef USE_THREADS
   while (1) {
     g_mutex_lock(cfc_data_mtx);
     while (!cfc_data.filter_text || cfc_data.state != CFC_PENDING) {
       /* Do we really need to use a mutex here? We only have one thread... */
       g_cond_wait(cfc_data_cond, cfc_data_mtx);
     }
-#endif
     cfc_data.state = CFC_UNKNOWN;
     DEBUG_SYNTAX_CHECK("pending", "unknown");
-#ifdef USE_THREADS
     g_mutex_unlock(cfc_data_mtx);
     g_mutex_lock(pcap_compile_mtx);
-#endif
+
     /* pcap_compile_nopcap will not alter the filter string, so the (char *) cast is "safe" */
     pc_err = pcap_compile_nopcap(DUMMY_SNAPLENGTH /* use a dummy snaplength for syntax-checking */,
             cfc_data.dlt, &fcode, cfc_data.filter_text, 1 /* Do optimize */,
             DUMMY_NETMASK /* use a dummy netmask for syntax-checking */);
-#ifdef USE_THREADS
+
     g_mutex_unlock(pcap_compile_mtx);
     g_mutex_lock(cfc_data_mtx);
-#endif
+
     if (cfc_data.state == CFC_UNKNOWN) { /* No more input came in */
       if (pc_err) {
         DEBUG_SYNTAX_CHECK("unknown", "known bad");
@@ -342,10 +337,8 @@ check_capture_filter_syntax(void *data _U_) {
         cfc_data.state = CFC_VALID;
       }
     }
-#ifdef USE_THREADS
     g_mutex_unlock(cfc_data_mtx);
   }
-#endif
   return NULL;
 }
 
@@ -355,9 +348,8 @@ update_capture_filter_te(gpointer data _U_) {
   if (!prefs.capture_syntax_check_filter)
     return TRUE;
 
-#ifdef USE_THREADS
   g_mutex_lock(cfc_data_mtx);
-#endif
+
   if (cfc_data.filter_text && cfc_data.filter_te) {
     if (cfc_data.state == CFC_VALID) {
       colorize_filter_te_as_valid(cfc_data.filter_te);
@@ -377,9 +369,7 @@ update_capture_filter_te(gpointer data _U_) {
       cfc_data.state = CFC_PENDING;
     }
   }
-#ifdef USE_THREADS
   g_mutex_unlock(cfc_data_mtx);
-#endif
   return TRUE;
 }
 
@@ -389,13 +379,11 @@ void capture_filter_init(void) {
   cfc_data.filter_text = NULL;
   cfc_data.filter_te = NULL;
   cfc_data.state = CFC_PENDING;
-#ifdef USE_THREADS
   pcap_compile_mtx = g_mutex_new();
   cfc_data_cond = g_cond_new();
   cfc_data_mtx = g_mutex_new();
   g_timeout_add(200, update_capture_filter_te, NULL);
   g_thread_create(check_capture_filter_syntax, NULL, FALSE, NULL);
-#endif
 }
 
 static void
@@ -431,9 +419,7 @@ capture_filter_check_syntax_cb(GtkWidget *w _U_, gpointer user_data _U_)
     return;
   }
 
-#ifdef USE_THREADS
   g_mutex_lock(cfc_data_mtx);
-#endif
   /* Ruthlessly clobber the current state. */
   if (cfc_data.filter_text != NULL) {
     g_free(cfc_data.filter_text);
@@ -442,21 +428,14 @@ capture_filter_check_syntax_cb(GtkWidget *w _U_, gpointer user_data _U_)
   cfc_data.filter_te = filter_te;
   cfc_data.state = CFC_PENDING;
   DEBUG_SYNTAX_CHECK("?", "pending");
-#ifdef USE_THREADS
   g_cond_signal(cfc_data_cond);
   g_mutex_unlock(cfc_data_mtx);
-#else
-  check_capture_filter_syntax(NULL);
-  update_capture_filter_te(NULL);
-#endif
 }
 
 static void
 capture_filter_destroy_cb(GtkWidget *w _U_, gpointer user_data _U_)
 {
-#ifdef USE_THREADS
   g_mutex_lock(cfc_data_mtx);
-#endif
   /* Reset the current state to idle. */
   if (cfc_data.filter_text != NULL) {
     g_free(cfc_data.filter_text);
@@ -464,9 +443,7 @@ capture_filter_destroy_cb(GtkWidget *w _U_, gpointer user_data _U_)
   cfc_data.filter_text = NULL;
   cfc_data.filter_te = NULL;
   cfc_data.state = CFC_PENDING;
-#ifdef USE_THREADS
   g_mutex_unlock(cfc_data_mtx);
-#endif
 }
 
 #define TIME_UNIT_SECOND    0
@@ -1638,18 +1615,14 @@ capture_filter_compile_cb(GtkWidget *w _U_, gpointer user_data _U_)
   pd = pcap_open_dead(dlt, DUMMY_SNAPLENGTH);
   filter_cm = g_object_get_data(G_OBJECT(opt_edit_w), E_CFILTER_CM_KEY);
   filter_text = gtk_combo_box_text_get_active_text (GTK_COMBO_BOX_TEXT(filter_cm));
-#ifdef USE_THREADS
     g_mutex_lock(pcap_compile_mtx);
-#endif
   /* pcap_compile will not alter the filter string, so the (char *) cast is "safe" */
 #ifdef PCAP_NETMASK_UNKNOWN
   if (pcap_compile(pd, &fcode, (char *)filter_text, 1 /* Do optimize */, PCAP_NETMASK_UNKNOWN) < 0) {
 #else
   if (pcap_compile(pd, &fcode, (char *)filter_text, 1 /* Do optimize */, 0) < 0) {
 #endif
-#ifdef USE_THREADS
     g_mutex_unlock(pcap_compile_mtx);
-#endif
     simple_dialog(ESD_TYPE_ERROR, ESD_BTN_OK, "%s", pcap_geterr(pd));
   } else {
     GString *bpf_code_dump = g_string_new("");
@@ -1799,11 +1772,7 @@ update_options_table(gint index)
 #else
     gtk_list_store_set (GTK_LIST_STORE(model), &iter, CAPTURE, TRUE, INTERFACE, temp,LINK, link->name,  PMODE, row.pmode?"enabled":"disabled", SNAPLEN, snaplen_string, FILTER, row.cfilter, -1);
 #endif
-#ifdef USE_THREADS
     if (num_selected > 0) {
-#else
-    if (num_selected == 1) {
-#endif
       gtk_widget_set_sensitive(ok_bt, TRUE);
     } else {
       gtk_widget_set_sensitive(ok_bt, FALSE);
@@ -2362,11 +2331,7 @@ static void toggle_callback(GtkCellRendererToggle *cell _U_,
   } else {
     gtk_widget_set_sensitive(pcap_ng_cb, TRUE);
   }
-#ifdef USE_THREADS
   if (num_selected > 0) {
-#else
-  if (num_selected == 1) {
-#endif
     gtk_widget_set_sensitive(ok_bt, TRUE);
   } else {
     gtk_widget_set_sensitive(ok_bt, FALSE);
@@ -2526,11 +2491,7 @@ void enable_selected_interface(gchar *name, gboolean enable)
       break;
     }
   }
-#ifdef USE_THREADS
   if (num_selected > 0) {
-#else
-  if (num_selected == 1) {
-#endif
     gtk_widget_set_sensitive(ok_bt, TRUE);
   } else {
     gtk_widget_set_sensitive(ok_bt, FALSE);
@@ -2574,11 +2535,7 @@ static void capture_all_cb(GtkToggleButton *button, gpointer d _U_)
   if (get_welcome_window() != NULL) {
     change_selection_for_all(enabled);
   }
-#ifdef USE_THREADS
   if (num_selected > 0) {
-#else
-  if (num_selected == 1) {
-#endif
     gtk_widget_set_sensitive(ok_bt, TRUE);
   } else {
     gtk_widget_set_sensitive(ok_bt, FALSE);
@@ -3234,11 +3191,7 @@ capture_prep_cb(GtkWidget *w _U_, gpointer d _U_)
   ok_bt = g_object_get_data(G_OBJECT(bbox), WIRESHARK_STOCK_CAPTURE_START);
   g_signal_connect(ok_bt, "clicked", G_CALLBACK(capture_start_cb), NULL);
   gtk_widget_set_tooltip_text(ok_bt, "Start the capture process.");
-#ifdef USE_THREADS
   if (num_selected > 0) {
-#else
-  if (num_selected == 1) {
-#endif
     gtk_widget_set_sensitive(ok_bt, TRUE);
   } else {
     gtk_widget_set_sensitive(ok_bt, FALSE);
diff --git a/gtk/capture_if_dlg.c b/gtk/capture_if_dlg.c
index 7afacaa1c6..959ee4360b 100644
--- a/gtk/capture_if_dlg.c
+++ b/gtk/capture_if_dlg.c
@@ -186,7 +186,7 @@ store_selected(GtkWidget *choose_bt, gpointer if_data)
       temp->selected ^=1;
       if_data_list = g_list_remove(if_data_list, curr->data);
       if_data_list = g_list_insert(if_data_list, temp, ifs);
-      
+
       for (i = 0; i < global_capture_opts.ifaces->len; i++) {
         if (strcmp(g_array_index(global_capture_opts.ifaces, interface_options, i).name, temp->if_info.name) == 0) {
           found = TRUE;
@@ -210,8 +210,8 @@ store_selected(GtkWidget *choose_bt, gpointer if_data)
 #endif
             break;
           }
-        } 
-      } 
+        }
+      }
       if (!found && temp->selected) {
         interface_opts.name = g_strdup(temp->if_info.name);
         interface_opts.descr = get_interface_descriptive_name(interface_opts.name);
@@ -248,7 +248,7 @@ store_selected(GtkWidget *choose_bt, gpointer if_data)
           enable_selected_interface(interface_opts.name, TRUE);
         }
       }
-      
+
       if (temp->selected)
         currently_selected += 1;
       else
@@ -257,11 +257,7 @@ store_selected(GtkWidget *choose_bt, gpointer if_data)
     }
   }
   if (cap_if_w) {
-#ifdef USE_THREADS
     gtk_widget_set_sensitive(capture_bt, !gbl_capture_in_progress && (currently_selected > 0));
-#else
-    gtk_widget_set_sensitive(capture_bt, !gbl_capture_in_progress && (currently_selected == 1));
-#endif
   }
 }
 
@@ -393,11 +389,7 @@ set_capture_if_dialog_for_capture_in_progress(gboolean capture_in_progress)
   gbl_capture_in_progress = capture_in_progress;
   if (cap_if_w) {
     gtk_widget_set_sensitive(stop_bt, capture_in_progress);
-#ifdef USE_THREADS
     gtk_widget_set_sensitive(capture_bt, !capture_in_progress && (currently_selected > 0));
-#else
-    gtk_widget_set_sensitive(capture_bt, !capture_in_progress && (currently_selected == 1));
-#endif
   }
 }
 
@@ -993,13 +985,13 @@ capture_if_cb(GtkWidget *w _U_, gpointer d _U_)
 #endif
 
     if_data_list = g_list_append(if_data_list, if_dlg_data);
-    
+
     row++;
     if (row <= 10) {
         /* Lets add up 10 rows of interfaces, otherwise the window may become too high */
       gtk_widget_get_preferred_size(GTK_WIDGET(if_dlg_data->choose_bt), &requisition, NULL);
       height += requisition.height;
-    } 
+    }
   }
 
   g_string_free(if_tool_str, TRUE);
@@ -1060,7 +1052,7 @@ void select_all_interfaces(gboolean enable)
     curr = g_list_nth(if_data_list, ifs);
     temp = (if_dlg_data_t *)(curr->data);
     update_selected_interface(temp->if_info.name, enable);
- } 
+ }
 }
 
 void destroy_if_window(void)
diff --git a/gtk/main.c b/gtk/main.c
index 3f5bda29c6..0caf80323d 100644
--- a/gtk/main.c
+++ b/gtk/main.c
@@ -2316,9 +2316,7 @@ main(int argc, char *argv[])
   optind = optind_initial;
   opterr = 1;
 
-#ifdef USE_THREADS
   g_thread_init(NULL);
-#endif
 
   /* Set the current locale according to the program environment.
    * We haven't localized anything, but some GTK widgets are localized
@@ -2781,12 +2779,6 @@ main(int argc, char *argv[])
   prefs_apply_all();
 
 #ifdef HAVE_LIBPCAP
-#ifndef USE_THREADS
-  if ((global_capture_opts.ifaces->len > 1) && start_capture) {
-    cmdarg_err("You specified multiple interfaces for capturing which this version of Wireshark doesn't support.");
-    exit(2);
-  }
-#endif
   if ((global_capture_opts.ifaces->len == 0) &&
       (prefs.capture_device != NULL)) {
     GList *curr, *combo_list;
diff --git a/gtk/main.h b/gtk/main.h
index 59b742ddfc..79fba33198 100644
--- a/gtk/main.h
+++ b/gtk/main.h
@@ -144,7 +144,7 @@ typedef enum {
  */
 extern void copy_selected_plist_cb(GtkWidget *w _U_, gpointer data, COPY_SELECTED_E action);
 
-/** Set or remove a time reference on this frame 
+/** Set or remove a time reference on this frame
  *
  * @param set TRUE = set time ref, FALSE=unset time ref
  * @param frame pointer to frame
@@ -346,7 +346,7 @@ extern gboolean main_filter_packets(capture_file *cf, const gchar *dftext,
 extern void create_console(void);
 #endif
 
-#if defined(_WIN32) || ! defined USE_THREADS
+#if defined(_WIN32)
 /** Restart the tap update display timer with new configured interval */
 extern void reset_tap_update_timer(void);
 #endif
diff --git a/gtk/main_welcome.c b/gtk/main_welcome.c
index 8226f5a588..c78457102a 100644
--- a/gtk/main_welcome.c
+++ b/gtk/main_welcome.c
@@ -111,9 +111,7 @@ static GArray *interfaces = NULL;
 
 static GSList *status_messages = NULL;
 
-#ifdef USE_THREADS
 static GMutex *recent_mtx = NULL;
-#endif
 
 /* The "scroll box dynamic" is a (complicated) pseudo widget to */
 /* place a vertically list of widgets in (currently the interfaces and recent files). */
@@ -503,9 +501,7 @@ static void *get_recent_item_status(void *data)
      * most OSes use.
      */
     err = ws_stat64(ri_stat->filename, &stat_buf);
-#ifdef USE_THREADS
     g_mutex_lock(recent_mtx);
-#endif
     ri_stat->err = err;
     if(err == 0) {
         if (stat_buf.st_size/1024/1024/1024 > 10) {
@@ -531,9 +527,7 @@ static void *get_recent_item_status(void *data)
     } else {
         ri_stat->stat_done = TRUE;
     }
-#ifdef USE_THREADS
     g_mutex_unlock(recent_mtx);
-#endif
 
     return NULL;
 }
@@ -549,9 +543,7 @@ update_recent_items(gpointer data)
         return FALSE;
     }
 
-#ifdef USE_THREADS
     g_mutex_lock(recent_mtx);
-#endif
     if (ri_stat->stat_done) {
         again = FALSE;
         gtk_label_set_markup(GTK_LABEL(ri_stat->label), ri_stat->str->str);
@@ -562,9 +554,7 @@ update_recent_items(gpointer data)
         ri_stat->timer = 0;
     }
     /* Else append some sort of Unicode or ASCII animation to the label? */
-#ifdef USE_THREADS
     g_mutex_unlock(recent_mtx);
-#endif
     return again;
 }
 
@@ -575,9 +565,7 @@ static void welcome_filename_destroy_cb(GtkWidget *w _U_, gpointer data) {
         return;
     }
 
-#ifdef USE_THREADS
     g_mutex_lock(recent_mtx);
-#endif
     if (ri_stat->timer) {
 	g_source_remove(ri_stat->timer);
 	ri_stat->timer = 0;
@@ -592,9 +580,7 @@ static void welcome_filename_destroy_cb(GtkWidget *w _U_, gpointer data) {
     } else {
         ri_stat->label = NULL;
     }
-#ifdef USE_THREADS
     g_mutex_unlock(recent_mtx);
-#endif
 }
 
 /* create a "file link widget" */
@@ -644,13 +630,8 @@ welcome_filename_link_new(const gchar *filename, GtkWidget **label, GObject *men
     g_signal_connect(w, "destroy", G_CALLBACK(welcome_filename_destroy_cb), ri_stat);
     g_free(str_escaped);
 
-#ifdef USE_THREADS
     g_thread_create(get_recent_item_status, ri_stat, FALSE, NULL);
     ri_stat->timer = g_timeout_add(200, update_recent_items, ri_stat);
-#else
-    get_recent_item_status(ri_stat);
-    update_recent_items(ri_stat);
-#endif
 
     /* event box */
     eb = gtk_event_box_new();
@@ -1022,14 +1003,14 @@ welcome_if_tree_load(void)
                     g_free (comment);
 #endif
                     gtk_list_store_set(store, &iter, ICON, gtk_image_get_pixbuf(GTK_IMAGE(icon)), IFACE_DESCR, user_descr, IFACE_NAME, if_info->name, -1);
-                    d_interface.descr = g_strdup(user_descr);               
+                    d_interface.descr = g_strdup(user_descr);
                     g_free (user_descr);
                 } else if (if_info->description) {
                     gtk_list_store_set (store, &iter, ICON, gtk_image_get_pixbuf(GTK_IMAGE(icon)), IFACE_DESCR, if_info->description, IFACE_NAME, if_info->name, -1);
                     d_interface.descr = g_strdup(if_info->description);
                 } else {
                     gtk_list_store_set (store, &iter, ICON, gtk_image_get_pixbuf(GTK_IMAGE(icon)), IFACE_DESCR, if_info->name, IFACE_NAME, if_info->name, -1);
-                    d_interface.descr = g_strdup(if_info->name); 
+                    d_interface.descr = g_strdup(if_info->name);
                 }
                 g_array_append_val(interfaces, d_interface);
             }
@@ -1084,13 +1065,6 @@ static void capture_if_start(GtkWidget *w _U_, gpointer data _U_)
       "You didn't specify an interface on which to capture packets.");
     return;
   }
-#ifndef USE_THREADS
-  if (global_capture_opts.ifaces->len > 1) {
-    simple_dialog(ESD_TYPE_ERROR, ESD_BTN_OK,
-      "You specified multiple interfaces for capturing which this version of Wireshark doesn't support.");
-    return;
-  }
-#endif
 
   /* XXX - remove this? */
   if (global_capture_opts.save_file) {
@@ -1237,19 +1211,11 @@ welcome_new(void)
         gtk_tree_view_column_set_visible(column, FALSE);
         selection = gtk_tree_view_get_selection(GTK_TREE_VIEW(if_view));
         gtk_tree_selection_set_mode(selection, GTK_SELECTION_MULTIPLE);
-#ifdef USE_THREADS
         item_hb = welcome_button(WIRESHARK_STOCK_CAPTURE_START,
             "Start",
             "Choose one or more interfaces to capture from, then <b>Start</b>",
             "Same as Capture/Interfaces with default options",
             (welcome_button_callback_t)capture_if_start, (gpointer)if_view);
-#else
-        item_hb = welcome_button(WIRESHARK_STOCK_CAPTURE_START,
-            "Start",
-            "Choose exactly one interface to capture from, then <b>Start</b>",
-            "Same as Capture/Interfaces with default options",
-            (welcome_button_callback_t)capture_if_start, (gpointer)if_view);
-#endif
         gtk_box_pack_start(GTK_BOX(topic_to_fill), item_hb, FALSE, FALSE, 5);
         welcome_if_tree_load();
         gtk_container_add (GTK_CONTAINER (swindow), if_view);
@@ -1416,9 +1382,7 @@ welcome_new(void)
                                           welcome_eb);
     gtk_widget_show_all(welcome_scrollw);
 
-#ifdef USE_THREADS
     recent_mtx = g_mutex_new();
-#endif
 
     return welcome_scrollw;
 }
diff --git a/gtk/prefs_capture.c b/gtk/prefs_capture.c
index e5e81ef5e4..8ea8aa6e3c 100644
--- a/gtk/prefs_capture.c
+++ b/gtk/prefs_capture.c
@@ -101,9 +101,6 @@ capture_prefs_show(void)
 {
 	GtkWidget	*main_tb, *main_vb;
 	GtkWidget	*if_cbxe, *if_lb, *promisc_cb, *pcap_ng_cb, *sync_cb, *auto_scroll_cb, *show_info_cb;
-#ifndef USE_THREADS
-	GtkWidget	*syntax_check_filter_cb;
-#endif
 	GtkWidget	*ifopts_lb, *ifopts_bt;
 	GList		*if_list, *combo_list;
 	int		err;
@@ -209,15 +206,6 @@ capture_prefs_show(void)
 	    !prefs.capture_show_info);
 	g_object_set_data(G_OBJECT(main_vb), SHOW_INFO_KEY, show_info_cb);
 
-#ifndef USE_THREADS
-	/* Syntax check capture filter */
-	syntax_check_filter_cb = create_preference_check_button(main_tb, row++,
-	    "Syntax check capture filter:",
-	    "Syntax check capture filter. Turn this off if you experience delay when writing capture filters.",
-	    prefs.capture_syntax_check_filter);
-	g_object_set_data(G_OBJECT(main_vb), SYNTAX_CHECK_FILTER_KEY, syntax_check_filter_cb);
-#endif
-
 	/* Show 'em what we got */
 	gtk_widget_show_all(main_vb);
 
diff --git a/gtk/prefs_taps.c b/gtk/prefs_taps.c
index 8f7cc4991d..c9db484f58 100644
--- a/gtk/prefs_taps.c
+++ b/gtk/prefs_taps.c
@@ -67,7 +67,7 @@ stats_prefs_show(void)
 
         /* Tap update gap in ms */
         tap_update_interval_te = create_preference_entry(main_tb, pos++,
-            "Tap update interval in ms:", 
+            "Tap update interval in ms:",
             "Determines time between tap updates.", max_visible_str);
         g_snprintf(update_interval_str, sizeof(update_interval_str), "%d", prefs.tap_update_interval);
         gtk_entry_set_text(GTK_ENTRY(tap_update_interval_te), update_interval_str);
@@ -77,7 +77,7 @@ stats_prefs_show(void)
 #ifdef HAVE_LIBPORTAUDIO
         /* Max visible channels in RTP Player */
         rtp_player_max_visible_te = create_preference_entry(main_tb, pos++,
-            "Max visible channels in RTP Player:", 
+            "Max visible channels in RTP Player:",
             "Determines maximum height of RTP Player window.", max_visible_str);
         g_snprintf(max_visible_str, sizeof(max_visible_str), "%d", prefs.rtp_player_max_visible);
         gtk_entry_set_text(GTK_ENTRY(rtp_player_max_visible_te), max_visible_str);
@@ -124,7 +124,7 @@ stats_prefs_fetch(GtkWidget *w _U_)
 void
 stats_prefs_apply(GtkWidget *w _U_)
 {
-#if defined(_WIN32) || ! defined USE_THREADS
+#if defined(_WIN32)
         reset_tap_update_timer();
 #endif
 }
@@ -133,4 +133,3 @@ void
 stats_prefs_destroy(GtkWidget *w _U_)
 {
 }
-
diff --git a/version_info.c b/version_info.c
index 791508f9b0..381c1a4b75 100644
--- a/version_info.c
+++ b/version_info.c
@@ -153,11 +153,6 @@ get_compiled_version_info(GString *str, void (*prepend_info)(GString *),
 #else /* HAVE_LIBCAP */
 	g_string_append(str, "without POSIX capabilities");
 #endif /* HAVE_LIBCAP */
-#ifdef USE_THREADS
-	g_string_append_printf(str, ", with threads support");
-#else
-	g_string_append_printf(str, ", without threads support");
-#endif
 
 	/* Additional application-dependent information */
 	if (append_info)