diff --git a/epan/prefs.c b/epan/prefs.c
index 0ca84c3636..4b993001ef 100644
--- a/epan/prefs.c
+++ b/epan/prefs.c
@@ -2337,10 +2337,16 @@ prefs_register_modules(void)
                        "Layout content of the pane 3",
                        (gint*)(void*)(&prefs.gui_layout_content_3), gui_layout_content, FALSE);
 
+    prefs_register_bool_preference(gui_layout_module, "packet_list_separator.enabled",
+                                   "Enable Packet List Separator",
+                                   "Enable Packet List Separator",
+                                   &prefs.gui_qt_packet_list_separator);
+
     prefs_register_bool_preference(gui_module, "packet_editor.enabled",
                                    "Enable Packet Editor",
                                    "Enable Packet Editor (Experimental)",
                                    &prefs.gui_packet_editor);
+
     /* Console
      * These are preferences that can be read/written using the
      * preference module API.  These preferences still use their own
@@ -2975,6 +2981,8 @@ pre_init_prefs(void)
     prefs.gui_layout_content_3       = layout_pane_content_pbytes;
     prefs.gui_packet_editor          = FALSE;
 
+    prefs.gui_qt_packet_list_separator = FALSE;
+
     if (!prefs.col_list) {
         /* First time through */
         for (i = 0; i < DEF_NUM_COLS; i++) {
diff --git a/epan/prefs.h b/epan/prefs.h
index cd6398560e..8794374de6 100644
--- a/epan/prefs.h
+++ b/epan/prefs.h
@@ -212,7 +212,7 @@ typedef struct _e_prefs {
   gchar       *saved_at_version;
   gboolean     unknown_prefs; /* unknown or obsolete pref(s) */
   gboolean     unknown_colorfilters; /* unknown or obsolete color filter(s) */
-  guint        gui_qt_language; /* Qt Translation language selection */
+  gboolean     gui_qt_packet_list_separator;
   gboolean     gui_packet_editor; /* Enable Packet Editor */
   gboolean     st_enable_burstinfo;
   gboolean     st_burst_showcount;
diff --git a/ui/qt/layout_preferences_frame.cpp b/ui/qt/layout_preferences_frame.cpp
index 12c220d69c..814803dd3a 100644
--- a/ui/qt/layout_preferences_frame.cpp
+++ b/ui/qt/layout_preferences_frame.cpp
@@ -47,6 +47,9 @@ LayoutPreferencesFrame::LayoutPreferencesFrame(QWidget *parent) :
     ui->layout4ToolButton->setStyleSheet(image_pad_ss);
     ui->layout5ToolButton->setStyleSheet(image_pad_ss);
     ui->layout6ToolButton->setStyleSheet(image_pad_ss);
+
+    pref_packet_list_separator_ = prefFromPrefPtr(&prefs.gui_qt_packet_list_separator);
+    ui->packetListSeparatorCheckBox->setChecked(pref_packet_list_separator_->stashed_val.boolval);
 }
 
 LayoutPreferencesFrame::~LayoutPreferencesFrame()
@@ -282,6 +285,13 @@ void LayoutPreferencesFrame::on_restoreButtonBox_clicked(QAbstractButton *)
     updateWidgets();
     pref_layout_content_3_->stashed_val.enumval = pref_layout_content_3_->default_val.enumval;
     updateWidgets();
+
+    ui->packetListSeparatorCheckBox->setChecked(pref_packet_list_separator_->default_val.boolval);
+}
+
+void LayoutPreferencesFrame::on_packetListSeparatorCheckBox_toggled(bool checked)
+{
+    pref_packet_list_separator_->stashed_val.boolval = (gboolean) checked;
 }
 
 /*
diff --git a/ui/qt/layout_preferences_frame.h b/ui/qt/layout_preferences_frame.h
index cc04b5dab3..5fe260df6d 100644
--- a/ui/qt/layout_preferences_frame.h
+++ b/ui/qt/layout_preferences_frame.h
@@ -49,6 +49,7 @@ private:
     pref_t *pref_layout_content_1_;
     pref_t *pref_layout_content_2_;
     pref_t *pref_layout_content_3_;
+    pref_t *pref_packet_list_separator_;
 
     void updateWidgets();
 
@@ -72,6 +73,7 @@ private slots:
     void on_pane3PacketBytesRadioButton_toggled(bool checked);
     void on_pane3NoneRadioButton_toggled(bool checked);
     void on_restoreButtonBox_clicked(QAbstractButton *button);
+    void on_packetListSeparatorCheckBox_toggled(bool checked);
 
 };
 
diff --git a/ui/qt/layout_preferences_frame.ui b/ui/qt/layout_preferences_frame.ui
index edd5abbb60..00d7906595 100644
--- a/ui/qt/layout_preferences_frame.ui
+++ b/ui/qt/layout_preferences_frame.ui
@@ -7,7 +7,7 @@
     <x>0</x>
     <y>0</y>
     <width>414</width>
-    <height>259</height>
+    <height>287</height>
    </rect>
   </property>
   <property name="windowTitle">
@@ -328,6 +328,29 @@
      </item>
     </layout>
    </item>
+   <item>
+    <spacer name="verticalSpacer_2">
+     <property name="orientation">
+      <enum>Qt::Vertical</enum>
+     </property>
+     <property name="sizeType">
+      <enum>QSizePolicy::Fixed</enum>
+     </property>
+     <property name="sizeHint" stdset="0">
+      <size>
+       <width>20</width>
+       <height>10</height>
+      </size>
+     </property>
+    </spacer>
+   </item>
+   <item>
+    <widget class="QCheckBox" name="packetListSeparatorCheckBox">
+     <property name="text">
+      <string>Show packet separator on Packet List</string>
+     </property>
+    </widget>
+   </item>
    <item>
     <spacer name="verticalSpacer">
      <property name="orientation">
diff --git a/ui/qt/packet_list.cpp b/ui/qt/packet_list.cpp
index 8aee922ace..96c3ae1408 100644
--- a/ui/qt/packet_list.cpp
+++ b/ui/qt/packet_list.cpp
@@ -442,6 +442,18 @@ PacketList::PacketList(QWidget *parent) :
             this, SIGNAL(editProtocolPreference(preference*,pref_module*)));
 }
 
+void PacketList::drawRow (QPainter *painter, const QStyleOptionViewItem &option, const QModelIndex &index) const
+{
+    QTreeView::drawRow(painter, option, index);
+
+    if (prefs.gui_qt_packet_list_separator) {
+        QRect rect = visualRect(index);
+
+        painter->setPen(QColor(Qt::white));
+        painter->drawLine(0, rect.y() + rect.height() - 1, width(), rect.y() + rect.height() - 1);
+    }
+}
+
 void PacketList::setProtoTree (ProtoTree *proto_tree) {
     proto_tree_ = proto_tree;
 
diff --git a/ui/qt/packet_list.h b/ui/qt/packet_list.h
index ae7b751281..67b9022d65 100644
--- a/ui/qt/packet_list.h
+++ b/ui/qt/packet_list.h
@@ -32,6 +32,7 @@
 #include <QMenu>
 #include <QTime>
 #include <QTreeView>
+#include <QPainter>
 
 class OverlayScrollBar;
 
@@ -81,6 +82,7 @@ protected:
 
 protected slots:
     void rowsInserted(const QModelIndex &parent, int start, int end);
+    void drawRow(QPainter *painter, const QStyleOptionViewItem &option, const QModelIndex &index ) const;
 
 private:
     PacketListModel *packet_list_model_;